Deep UV-microscopy down to 230nm
Standard microscopes do not transmit light below ca. 320nm. Both, the objective and the other lenses in a microscope absorb light at these wavelengths. For applications like
- imaging at e.g. 260nm
- fluorescence excitation below 320nm
- photoactivation
- measurement of spatially resolved absorption spectra in the UV (OSD)
we offer solutions.
Solution:
1. Use of quartz objective
2. Modification of the fluorescence excitation optical pathway.
3. Use of suitable dichroic mirrors in the UV
4. Suitable light sources
5. UV-sensitive cameras
Visualization of modified microscope light path (magenta) to pass UV, (yellow pathway unmodified):
Deep UV light sources are introduced to the microscope via light guide and special UV condensors (LGA-Z/UV).
Modification of other microscopes on request.
Available UV quartz objectives:
10x, NA 0.2, working distance 7.4mm
20x, NA 0.65 air, working distance 2.0mm
40x, NA 0.8 air, workling distance 0.8mm
40x, NA 0.25 glyc, working distance 0.5mm